Ụmụ irighiri ihe nke ọbara na-edozi site electrophoresis

Ahụ mmadụ - a mgbagwoju usoro nke ọ bụla nke abụọ e nwere dị iche iche chemical Filiks: na homonụ emepụta fractions ọbara na-edozi, pigments, wdg Na mmepe nke nkà mmụta ọgwụ ghọrọ dị ka ndị mmadụ, ọtụtụ ndị ọmụmụ iji chọpụta larịị nke dị bekee ... Abnormalities nwere ike na-egosi na mmepe nke ọrịa, ma ọ bụ ịbụ n'ihi n'ahụ ihe.

ozi izugbe

E nwere ọtụtụ ọnọdụ nke onye fractions ọbara protein ụba. Gịnị ka ọ bụ, ihe nwere ike na-egosi yiri ọgụgụ, ole na ole maara.

Ndị bekee na ihe akụkụ nke ọbara plasma na iso nke mgbagwoju amino asịd.

Igosi nnọọ mkpa ọrụ:

1. Ebu mkpa bekee (nke nwere ike mejuputara ọgwụ, lipids, vitamin, minerals, pigments na dị ka. D.).
2. Maka clotting.
3. Ịnọgide na-enwe acid-alkaline itule.
4. Gbanwee nke itule nke leukocytes, erythrocytes na platelet.
5. Emetụta ala nke ọgụ.

Ha larịị na-adabere na-anọgidesi nke ọbara. Na ok na viscous mmiri mmiri njọ gafee site n'ọbara, si otú na-akawanye njọ ọrụ dum obi usoro.

Iji chọpụta protein nta a rụrụ ad hoc analysis - proteinogramma.

Biochemical analysis of ọbara

Nke a laabu ọmụmụ bụ otu n'ime ndị kasị ihe ọmụma na-ewu ewu. Ọ họpụtara fọrọ nke nta niile ọrịa n'ịnwụ inpatient ọgwụgwọ.

Ọ-enye gị ohere na-amata na n'ozuzu ọnọdụ nke ahụ na ọrụ dị iche iche na akụkụ dị iche iche kwa.

protein nta na Biochemical analysis of ọbara bụ akụkụ dị oké mkpa, nke a rụrụ maka mmadụ niile na n'ihe ize ndụ n'ihi na ụfọdụ ọrịa dị iche iche.

Iji gosi

Ọtụtụ mgbe, ndị na-amụ nke protein fractions kenyere enyo enyo daa ọrịa dị ka ndị a:

• myeloma;
• monoclonal gammopathy.

Iduziri proteinogram gosiri na ọrịa na:

• autoimmune Filiks (scleroderma, lupus erythematosus, Hashimoto tireoditom);
• -adịghị ala ala na nnukwu na-efe efe (herpes, ịba ọcha n'anya nke niile ụdị, Epstein-Barr virus na cytomegalovirus);
• malobsorbtsii mgbaàmà (-adịghị ala ala afọ mgbu, na-egosipụta site na afọ ọsịsa, steatorrhea, nke iduga malabsorption nke nri);
• nsogbu iri (enweghị ma ọ bụ na-abawanye na agụụ, agụụ na-agụ n'ihi na ntuli aka ụfọdụ ngwaahịa).

Ọzọkwa, ọ na ike ga-ndinọ na a ngụkọta nnyocha ahu (echetakwa nke dị iche iche ọrịa).

A mkpebi na mkpa maka nnyocha ga-dọkịta (general eme) na ndabere nke mkpesa, akụkọ ihe mere eme nke onye ọrịa, ule results na otú pụta.

analysis Atụmatụ

All ọrịa ndị họpụtara ọmụmụ fractions nke na-edozi na ọbara, mkpa ka ị mara:

1. Usoro a rụrụ na ihe efu afo n'isi-ututu. Tupu mmalite nke nnyefe na-adịghị na-eri n'ihi na 8 awa.
2. Na eve nke na-atụ aro usoro iri nri (ewepu mmanya, ọdụdụ, na-ese siga, e ghere eghe, na-atọkwa ụtọ, nri ngwa ngwa, Mayonezi, tomato kechoopu na ndị ọzọ na-emerụ ngwaahịa). Ọ bụ ihe amamihe amachi oriri nke protein.
3. 30-60 min. dịghị ise siga.
4. Zere Onyonyo ọmụmụ: x-ụzarị, X-ụzarị, ultrasound, wdg ruo ọtụtụ ụbọchị tupu analysis ...
5. Na eve nwere ike ịbụ ụjọ na-eme ike, imega ahụ.
6. Obere ụmụaka teething usoro a na-adịghị rụrụ.

Mebiri nke ọ bụla ihe si ndepụta n'elu ike ịkpaghasị n'ihi, tinye ọrịa na dọkịta ahụ na-eduhie eduhie. Ya mere, ọ dị mkpa ịgbaso a ndepụta kwe nkwa.

Olee ihe ọzọ nwere ike ime ka a ndiiche na-arụpụta:

• igba ogwu n'akwara infusion ngwọta;
• oké sweating;
• ime;
• lactation;
• ibu ibu dị arọ;
• bed ọzọ;
• ịṅụ nke a protein nri.

nchọpụta ụzọ

Ụmụ irighiri ihe nke na-edozi na-akụkụ nke ngụkọta protein. Nke ikpeazụ a na-kpebisie ike site na-eduzi n'elu-kọwara Biochemical ọbara analysis. N'ihi na ihe ọmụma zuru ezu ntule nke itule nke ya ahụ nwere dị iche iche ọmụmụ.

The kasị ihe ọmụma na-ewu ewu na Usoro electrophoresis, nke nwere ike mere site na iji a pụrụ iche ihe:

• hromatogroficheskoy akwụkwọ;
• cellulose acetate film;
• starch ma ọ bụ agar jel.

Ọ na-adị site n'oru nke ihe eletrik na ọbara ndi na-edozi na-enịm na a pụrụ iche akwụkwọ ma ọ bụ a jel. Na usoro nke-enweta a na-ezighị ezi-elekọta mmadụ, nke n'okpuru nduzi nke iwu nke physics na-kwagara anode na nwere ike iche na 6 dị iche iche ụmụ irighiri ihe, ya bụ:

• albumin;
• globulin (Alfa-1 na Alfa-2);
• globulins (beta na gamma);
• prealbumin.

Ke adianade na-ekpebi ọbara protein fractions site electrophoresis, lab technicians ike ga-eji na ndị ọzọ na-erughị na-ewu ewu ụzọ:

• jel filtration;
• immunoassay;
• ọdịda;
• sedimentation.

Norms na deviations

The analysis na rụrụ ngwa ngwa-ezuru, na n'ihi pụrụ inweta na ndị na-esonụ ụbọchị.

On arụmọrụ nke ụmụ irighiri ihe nke na-edozi na-enwe mmetụta na ọtụtụ ihe. The nkezi ego nke nkịtị ọbara kwesịrị (g / DL):

• Albumin - 3,5-5,00;
• A-1 - 0.1-0.3;
• a-2 - 0,6-1,00;
• beta - 0.7-1.2;
• gamma - 0.7-1.6.

Ngụkọta protein ruru kwesịrị ịbụ na nso 6,4-8,3 g / DL.

Nso ke abawanye

ọbara protein fractions kwesịrị ịbụ na a kpọmkwem ruru, nke e ji mara izugbe ọnọdụ nke ahụ mmadụ.

Mebiri nke a itule Ndị Hapụrụ ọrịa dysproteinemia dịrị gaba n'ime ebe.

Dabere na mgbanwe nke a akpan akpan òtù nwere ike ikwu banyere ụfọdụ pathologies, obi kpamkpam ma ọ bụ n'ụzọ doro anya na-ewere ọnọdụ ke idem. Ọ bụrụ na elu etoju:

• albumin - nke a nwere ike na-egosi umeji akụrụ ọrịa; e.g., ịba ọcha n'anya, imeju, carcinoma, pyelonephritis na ndị ọzọ;
• globulins nke Alfa 1 (2) - ike na-egosi na ọnụnọ nke mbufụt, oké nchegbu ma ọ bụ ndị na-agba ọsọ na ogbo nke cancer metastasis;
• beta - ke idem nwere ike ịbụ ugbu hyperlipoproteinemia (lipid metabolism);
• Gamma - uto ọnụego bụ ihe e ji mara maka penetration nke ibute ọrịa (nje ma ọ bụ malitere ịrịa).

Ọ bụrụ na n'oge proteinogram na ule warara achọpụtara ndiiche monoclonal protein, ọtụtụ mgbe, nke a bụ ihe mere chee myeloma - a cancer of plasma mkpụrụ ndụ. Ya mere, mgbe na-eduzi a nnyocha e mere na daa ọrịa proteinogramma na-kenyere otu n'ime ndị kasị oké ọnụ ahịa na ihe ọmụma ụzọ.

Derated

Ọtụtụ ndị ọrịa nwere ike na-ahụ na ha na-arụpụta a ọnụ ke ngụkọta protein na ya onye fractions. Nke a pụrụ ịbụ a otu ụkpụrụ na-enweghị nchịkwa na ndiiche.

Na steeti a na-akpọ hypoproteinemia. Ọ nwere ike triggered site na:

• ezughi oke protein oriri na nri;
• ogologo nri;
• anaemia;
• menses;
• postoperative oge;
• fever;
• na-efe efe Filiks;
• igbu egbu;
• akụrụ ọrịa;
• ịba ọcha n'anya;
• enweghị ụra;
• n'ozuzu ọnwụ nke ume;
• ọbara ọgbụgba.

N'ihi na n'ahụ ihe (.. ntụgharị, na-abụghị ọrịa) budata ibelata protein n'ime ọbara (n'okpuru 50 g / l) na-agụnye:

• lactation;
• ikpeazụ trimester nke dị ime;
• emegaghị, na N'ihi ya nke a ogologo ọnụnọ ke ndina;
• oké emega ahụ.

Gịnị na-eme mgbe a ọjọọ n'ihi

ga ozugbo masịrị mgbe anata ogbenye arụmọrụ analysis na protein fractions. Ị ga ịkpọ dọkịta gị ma na-agbalị icheta na pụrụ ịkpata ndị dị otú ahụ a na steeti.

Dabere na-anakọtara akụkọ ihe mere eme na dọkịta ga-nye ndụmọdụ ka na-ekpeghachi analysis ọzọ mgbe e mesịrị ma ọ bụ inye onye ọzọ nnyocha.

Mbụ niile na ọ ga-adị mkpa rue:

• n'ozuzu na zuru ọbara ọnụ;
• urinalysis.

Instrumental ụzọ ga-họpụtara na ndabere nke mkpesa nke onye ọrịa. Ka ihe atụ, ọ bụrụ na na a ndabere nke mgbanwe nke albumin etoju ke ọrịa afo ga-afụ ụfụ, mgbe ahụ ọ ga-mkpa na-ebu ultrasound nke afo, x-ray nke ebe na na. N.

Dị ka ọgwụ na ahụ ike omume, dịpụrụ adịpụ abawanye ngụkọta protein na ọbara bụ mgbe a na-ahụkarị otu a karịa na shiee ụmụ irighiri ihe-ya nile. Ọnọdụ ndị dị otú na-achọ a ọzọ nyochaa ma na-amụ ihe ndị mere onye ọrịa. Ọ bụrụ na mmadụ na-ele onwe ya, o nwere ihe mgbu na ndị ọzọ chọpụtara na iji, na elu puru anyị nwere ike ikwu na ọ bụ ike. Na obere okwu, oké n'oge nkebi nke ọrịa ndị dị ka ọrịa cancer, imeju na m. P., On a ndabere anya wellbeing akpata mgbanwe ụfọdụ protein fractions. Ya mere, ọ bụla ndiiche si norm banyere mkpa ntị ha ike.