Protein denaturation

Conformation (Ọdịdị) nke peptide yinye na-nyere iwu na bụ pụrụ iche ọ bụla protein. Na pụrụ iche na ọnọdụ e nwere a ọdịiche ukwuu nke nkekọ nke nụ gbasara ohere Ọdịdị nke ahụ compound molekul. N'ihi ya, na ọdịiche kpamkpam dum molekul (ma ọ bụ a ọkpụrụkpụ òkè ya) na-ewe n'ụdị random eriri igwe. Usoro a na-akpọ "denaturation". Mgbanwe a nwere ike ịkpalite okpomọkụ si na iri isii na iri asatọ degrees. N'ihi ya, onye ọ bụla molekul, na n'ihi ọdịiche nwere ike iche na conformation si ọzọ.

Denaturation nke na-edozi na-ewe ebe n'okpuru nduzi nke ọ bụla mmadụ ike na-ebibi ndị na-abụghị covalent agbụ. Usoro a nwere ike ime n'okpuru alkaline ma ọ bụ acidic ọnọdụ, n'elu nke na-adọ ngalaba, n'okpuru ihe nke ụfọdụ organic ogige (phenols, alcohols na ndị ọzọ). protein denaturation nwere ike ime n'okpuru nduzi nke guanidinium chloride ma ọ bụ urea. Sị na mmadụ na-etolite na-adịghị ike agbụ (hydrophobic, ionic, hydrogen) na carbonyl ma ọ bụ amino iche iche nke peptide idé na a ọnụ ọgụgụ nke dị iche iche nke amino acid residues, nọ n'ọnọdụ ha nke dị protein hydrogen nkekọ n'ime ụmụ irighiri. N'ihi ya, a mgbanwe pụtara na sekọndrị na tertiary Ọdịdị.

Iguzogide denaturing mmadụ na-adabere tumadi na ọnụnọ na molekul nke protein ogige nke disulfide agbụ. Trypsin inhibitor nwere atọ nkekọ S - S. N'okpuru ọnọdụ nke mgbake nke protein denaturation emee enweghị ọzọ mmetụta. Ọ bụrụ na ekemende etinye njikọ ọnọdụ ebe ọxịdashọn a rụrụ SH-iche iche nke cysteine na guzobere disulfide agbụ, mbụ conformation na-weghachiri eweghachi. Ọnụnọ nke ọbụna otu disulfide nkekọ budata enwekwu ndị kwụsiri ike nke gbasara ohere Ọdịdị.

protein denaturation, a na-esonyere a ọnụ ke solubility. Ọnụ na a ịkpali mgbe iche-iche. Ọ na-adị n'ụdị "curdled protein." Na elu kasịnụ nke ogige na ngwọta, "coagulation" dabara dum solution, dị ka ihe atụ, a na-eme mgbe esi àkwá. Mgbe denaturation nke protein tụfuru ya ndu ọrụ. Ụkpụrụ a dabeere n'iji carbolic acid (phenol okenye ihe ngwọta) dị ka ihe antiseptik umi.

Akwusighi nke gbasara ohere Ọdịdị, akwa puru ọdịda n'okpuru nduzi nke dị iche iche mmadụ bụ budata ike na-anọrọ na protein nnyocha. Ọzọkwa kere ụfọdụ nsogbu mgbe iji ogige ke nkà mmụta ọgwụ na ụlọ ọrụ.

Ọ bụrụ na ndị protein denaturation e rụrụ site n'ebe okpomọkụ, ngwa ngwa jụrụ-ewe ebe ụfọdụ ọnọdụ usoro renativatsii - mgbake nke nwa afọ (mbụ) conformation. Eziokwu a na-egosi na ibikwasị nke peptide yinye bụ dị na isi Ọdịdị.

Guzobe nke nwa afọ conformation (isi ebe) bụ a n'amaghi usoro. Ndị ọzọ okwu, ndokwa a kwekọrọ na kacha nta ego nke free ike dị na a molekul. Otu nwere ike dị ka a N'ihi ga-kwubiri na ndị gbasara ohere Ọdịdị nke ahụ compound na-koodu na amino acid usoro na peptide ígwè. Nke a na n'aka pụtara na niile polypeptides metụtara site eri oké nri nke amino asịd (atụ, mioglobinovye peptide ígwè), ga-ihe yiri conformation.

Ndi na-edozi nwere ike ịrịba iche dị na isi Ọdịdị, ọbụna ma ọ bụrụ na obere ma ọ bụ nnọọ yiri na conformation.